Coding Regions of MSX1 Do Not Contribute to Non-Syndromic Cleft Lip With/Without Palate in Turkish Patients

Korkut Ulucan, Arzu Akcay, Burak Aksoy, Mehmet Boyraz, Deniz Kirac, Deniz Ergec, Necati Taskin, Ozhan Ozcelebiler, Muhsin Konuk, Teoman Akcay, A. Ilter Guney

Abstract


Background: One of the candidate genes for non-syndromic cleft lip with/without palate (NS-CL/P) is muscle segment homeobox 1 (MSX1) gene. MSX1 codes for a homeodomain protein that function as a transcriptional repressor during craniofacial development. In this study, we investigated the contribution of coding regions and exonic-intronic boundaries of MSX1 in Turkish NS-CL/P patients and the gender distribution of NS-CL/P in examined cohort.

Methods: We analyzed a total of 100 NS-CL/P Turkish patients, of whom 61 were boy and, 100 non-affected children of whom 44 were boys. Genotyping procedure was assessed by polymerase chain reaction-sequencing protocol. Fisher Exact Analysis was performed to compare the gender difference.

Results: We detected no variations in coding regions and intronic-exonic boundaries of MSX1 in both groups. Boys to girls ratio were 1.6 in our patient group. There was a statistically significant gender difference between groups, boys were more effected than girls (P = 0.023).

Conclusions: This was the first report including MSX1 in Turkish patients with NS-CL/P. We detected no sequence variations in the coding regions, including the exonic-intronic boundaries of MSX1 in our study population. More comprehensive analysis of MSX1 in Turkish population is required to fulfill MSX1 role for the onset of the anomaly.




Int J Clin Pediatr. 2014;3(1):12-15
doi: http://dx.doi.org/10.14740/ijcp136e


Keywords


Cleft lip with/without palate; Congenital; MSX1; Multifactorial; Orofacial clefts

Full Text: HTML PDF
 

Browse  Journals  

     

Journal of Clinical Medicine Research

Journal of Endocrinology and Metabolism

Journal of Clinical Gynecology and Obstetrics

World Journal of Oncology

Gastroenterology Research

Journal of Hematology

Journal of Medical Cases

Journal of Current Surgery

Clinical Infection and Immunity

Cardiology Research

World Journal of Nephrology and Urology

Cellular and Molecular Medicine Research

Journal of Neurology Research

International Journal of Clinical Pediatrics

 

 

 

 

 

International Journal of Clinical Pediatrics, quarterly, ISSN 1927-1255 (print), 1927-1263 (online), published by Elmer Press Inc.                     
The content of this site is intended for health care professionals.
This is an open-access journal distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, which permits unrestricted
non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Creative Commons Attribution license (Attribution-NonCommercial 4.0 International
CC-BY-NC 4.0)


This journal follows the International Committee of Medical Journal Editors (ICMJE) recommendations for manuscripts submitted to biomedical journals,
the Committee on Publication Ethics (
COPE) guidelines, and the Principles of Transparency and Best Practice in Scholarly Publishing.

website: www.theijcp.org   editorial contact: editor@theijcp.org    elmer.editorial2@hotmail.com
Address: 9225 Leslie Street, Suite 201, Richmond Hill, Ontario, L4B 3H6, Canada

© Elmer Press Inc. All Rights Reserved.



Disclaimer: The views and opinions expressed in the published articles are those of the authors and do not necessarily reflect the views or opinions of the editors and Elmer Press Inc. This website is provided for medical research and informational purposes only and does not constitute any medical advice or professional services. The information provided in this journal should not be used for diagnosis and treatment, those seeking medical advice should always consult with a licensed physician.